KARAKTERISASI EKSTRAK KASAR FITASE TERMOFILIK DARI BAKTERI KAWAH IJEN BANYUWANGI, ISOLAT AP-17

KARAKTERISASI EKSTRAK KASAR FITASE TERMOFILIK DARI BAKTERI KAWAH IJEN BANYUWANGI, ISOLAT AP-17

  • Aline Puspita Kusumadjaja Jurusan Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Negeri Surabaya
  • Tutuk Budiati Fakultas Farmasi Universitas Airlangga Surabaya
  • Sajidan Jurusan Pendidikan Matematika dan Ilmu Pengetahuan Alam Fakultas Keguruan dan Ilmu Pendidikan Universitas Negeri Sebelas Maret
  • Ni Nyoman Tri Puspaningsih Jurusan Kimia Fakultas Sain dan Teknologi Universitas Airlangga Surabaya
DOI: https://doi.org/10.23869/277
Keywords: Bacillus sp., AP-17, crude thermophilic phytase, optimum temperature, optimum pH

Abstract

Crude thermophilic phytase was produced by isolate AP-17 that has been isolated from Ijen Crater Banyuwangi. Based on Gram test, isolate AP-17 was gram positive spore forming rod shape bacteria so that it was identified as Bacillus sp. AP-17. Crude thermophilic phytase isolated from Bacillus sp. AP-17 had the optimum temperature at 75° C with activity of 0.1413 U/ml, and its optimum pH was at pH 6 with activity of 0.0875 U/ml. The enzyme was stable when heated at 75° C for three hours and still had 90% activity when it was exposed at pH 5–8, optimum temperature, for one hour.

Published
2010-12-01
How to Cite
Kusumadjaja, A. P., Budiati, T., Sajidan, & Puspaningsih, N. N. T. (2010). KARAKTERISASI EKSTRAK KASAR FITASE TERMOFILIK DARI BAKTERI KAWAH IJEN BANYUWANGI, ISOLAT AP-17. BERKALA PENELITIAN HAYATI JOURNAL OF BIOLOGICAL RESEARCHES, 16(1), 9-14. https://doi.org/10.23869/277
Issue
Vol 16 No 1 (2010): December 2010
Section
Articles