EMBRIOGENESIS SOMATIK ANGGREK BULAN Phalaenopsis amabilis (L.) Bl: STRUKTUR DAN POLA PERKEMBANGAN

EMBRIOGENESIS SOMATIK ANGGREK BULAN Phalaenopsis amabilis (L.) Bl: STRUKTUR DAN POLA PERKEMBANGAN

  • Edy Setiti Wida Utami Laboratorium Biologi Reproduksi, Fakultas MIPA UNAIR
  • Issirep Soemardi Laboratorium Anatomi Tumbuhan, Fakultas Biologi UGM
  • Taryono Taryono Laboratorium Kultur Jaringan Tumbuhan, Fakultas Pertanian UGM
  • Endang Semiarti Laboratorium Kultur Jaringan Tumbuhan, Fakultas Biologi UGM
Keywords: moon orchid, somatic embryogenesis, structure and developmental pattern, light microscopy

Abstract

Research of the structure and development pattern of somatic embryos from callus of leaf explants moon orchid Phalaenopsis amabilis (L) Bl had been done. One year old of plantlets were used as explants sources. Basal leaf of these explants were cultured in Somatic Embryo Induction Medium (SEIM) e.i.: NP(New Phalaenopsis) medium added with 2 mg/L NAA, 1 mg/L BA, 10 g/L sucrose, and 2 g/L gellan gum. Then somatic embryos were transferred to EMM (Embryo Maturation Medium) e.i.: NP medium added with 1 mg/L NAA, 1 mg/L BA, 10 g/L sucrose, and 2 g/L gellan gum. Finally, mature somatic embryo were transferred to NP medium without plant growth regulator as Embryo Germination Medium (EGM). The origin of somatic embryos initially from single cell at the pheriphery of embryogenic callus. These cells then devided in mitotic repeatedly formed globular proembryo, elongation embryo, and completed embryo. The structure and development pattern of somatic embryos as the same as with zygotic embryo.

Published
2012-02-05
How to Cite
Utami, E. S. W., Soemardi, I., Taryono, T., & Semiarti, E. (2012). EMBRIOGENESIS SOMATIK ANGGREK BULAN Phalaenopsis amabilis (L.) Bl: STRUKTUR DAN POLA PERKEMBANGAN. BERKALA PENELITIAN HAYATI JOURNAL OF BIOLOGICAL RESEARCHES, 13(1), 33-38. https://doi.org/10.23869/320