HIDROLISIS BEBERAPA JENIS XILAN DENGAN ENZIM XILANOLITIK TERMOFILIK REKOMBINAN:

Ni Nyoman Tri Puspaningsih; Hery Suwito; Sri Sumarsih; Ali Rohman; One Asmarani

doi:10.23869/353

Ni Nyoman Tri Puspaningsih Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Airlangga
Hery Suwito Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Airlangga
Sri Sumarsih Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Airlangga
Ali Rohman Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Airlangga
One Asmarani Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Airlangga

Keywords: commercial xylan, pNP-X, pTP510, xylanolytic enzyme

Abstract

The aims of this research were to know the ability of recombinant xylanolytic enzyme from recombinant E. coli DH5a (pTP510) to hydrolyze several commercial xylan and analysis the reduction sugar product. Recombinant xylanolytic enzyme (exo-xylanase, b-xylosidase and a-L-arabinofuranosidase) could hydrolyzed several commercial xylan (oat-spelt xylan, birchwood, wheat, rye, and arabinan) with xylanolytic activities are: oat-spelt xylan (1.73 U/mL), birchwood (0.92 U/mL), wheat (6.52 U/mL), rye (4.94 U/mL), and arabinan (3.40 U/mL). Xylanolytic enzyme assay use specific substrate p-nitrophenyl-b-D-xylopyranoside (pNP-X) shown xylosidase activity 15.869 U/mL. Hydrolysis product was analyzed by HPLC. The results showed that xylose, arabinose, and xylo-oligosaccharide were produced from birchwood, wheat, rye, and arabinan hydrolysis, although xylose and arabinose were produced from hydrolysis of oat-spelt xylan.