PERAN ANTIBODI KUNING TELUR (IgY) SEBAGAI OPSONIN UNTUK PENCEGAHAN SERANGAN MUTAN STREPTOCOCCUS SEROTIPE D (STREPTOCOCCUS SOBRINUS):

Okti Nadia Poetri; Retno D. Soejoedono; Agustin Indrawati; I Wayan T. Wibawan

doi:10.23869/359

Okti Nadia Poetri Bagian Mikrobiologi Medik, Fakultas Kedokteran Hewan Institut Pertanian Bogor
Retno D. Soejoedono Bagian Mikrobiologi Medik, Fakultas Kedokteran Hewan Institut Pertanian Bogor
Agustin Indrawati Bagian Mikrobiologi Medik, Fakultas Kedokteran Hewan Institut Pertanian Bogor
I Wayan T. Wibawan Bagian Mikrobiologi Medik, Fakultas Kedokteran Hewan Institut Pertanian Bogor

Keywords: immunoglobulin Y (IgY), streptococcus sobrinus, phagositosis

Abstract

The aim of this study was to explore the role of serotype d Mutan Streptococcus (Streptococcus sobrinus) spesific immunoglobulin Y (IgY-Ss) as opsonin against the same strain. The eggs were collected from Single Comb Brown Leghorn which has been immunized with Streptococcus sobrinus. Agar gel precipitation test was applied to detect IgY-Ss in serum and egg. Egg containing IgY-Ss was collected and extracted by PEG-Amonium sulphate and purified using fast protein liquid chromatography. The purity of IgY-Ss was determined by UV spectrophotometer. Molecular weight was established by SDS-PAGE (sodium dedocyl sulphate-poly acrilamide gel electrophoresis). Biological activities of IgY-Ss as opsonin was determined by phagocytosis assay. Phagositic activity of macrophages was not increased by preincubation of both S. sobrinus (107 CFU/ml) and 100 μg of IgY-S, however the phagositic capacity was increased from 1.6 bacterial cell/ macrophag to 5.17 bacterial cell/ macrophag. These finding suggest that IgY-Ss obtained from hens immunized with S. sobrinus provide an alternative to prevent S. sobrinus infection.