ISOLASI DAN ANALISIS GEN YANG RESPONSIF TERHADAP CEKAMAN KEKERINGAN PADA TEBU:

Wiwit Budi  Widyasari; Bambang  Sugiharto; Cahya Ismayadi; Kabul  Wahjudi; Untung  Murdiyatmo

doi:10.23869/417

Wiwit Budi Widyasari Pusat Penelitian Perkebunan Gula Indonesia, Jl. Pahlawan 25 Pasuruan
Bambang Sugiharto FMIPA-Biologi Universitas Jember, Jember
Cahya Ismayadi Pusat Penelitian Perkebunan Kopi dan Kakao, Jl. PB. Sudirman Jember
Kabul Wahjudi Pusat Penelitian Perkebunan Gula Indonesia, Jl. Pahlawan 25 Pasuruan
Untung Murdiyatmo Pusat Penelitian Perkebunan Gula Indonesia, Jl. Pahlawan 25 Pasuruan

Keywords: Saccharum officinarum, drought inducible protein, dip22-cDNA, RT-PCR

Abstract

This study was aimed to isolate and identify a gene that was responsive to drought stress in sugar cane variety M442-51, a variety that is widely used in pastures or dry land. Under drought stress, M442-51 showed an enhance expression of several proteins. One of the protein, namely dip22, (drought inducible protein, with a molecular weight of 22KDa) was isolated and analyzed. Amino acid sequence of this protein showed some homology with several stressed related proteins (OsAsr 1 from Oryza sativa leaves, ZM Bss1 of Zea mays leaves, LeAsr 1, LeAsr 2 and LeAsr 3 of Lycopersicum lycopersicon leaves). The 343 bp of dip22-cDNA fragment was obtained using RT PCR from mRNA of sugar cane leaves that has been exposed to drought stress. The results indicated that the gene dip22 plays an important role as a regulatory protein that are usually nested within cytoplasmic matrix or in the nucleus.