PENGARUH EKSTRAK AKAR GINGSENG JAWA DALAM BERBAGAI KADAR TERHADAP SINTESIS PROTEIN SERUM TIKUS

PENGARUH EKSTRAK AKAR GINGSENG JAWA DALAM BERBAGAI KADAR TERHADAP SINTESIS PROTEIN SERUM TIKUS

  • Tri Nurhariyati Jurusan Biologi, Fakultas MIPA, Universitas Airlangga, Surabaya
  • Dwi Winarni Jurusan Biologi, Fakultas MIPA, Universitas Airlangga, Surabaya
  • Salamun Salamun Jurusan Biologi, Fakultas MIPA, Universitas Airlangga, Surabaya
  • Y Sri Wulan Manuhara Jurusan Biologi, Fakultas MIPA, Universitas Airlangga, Surabaya
  • Saikhu Ahmad Husen Jurusan Biologi, Fakultas MIPA, Universitas Airlangga, Surabaya
Keywords: Talinum paniculatum Gaertn, protein synthesis, serum protein

Abstract

The aim of this research was identifying the stimulation serum protein synthesis following the treatment of Java Gingseng (Talinum paniculatum Gaertn) root extract in varying concentration. This research used 12 male rats of 8-9 weeks, divided into 4 groups. First group as control, second group to five group were administratered with Java gingseng root extract equal with 3.5, 10.5, and 21 mg dry roots / 100g BW / day per oral for 14 days. Blood serum was collected for electrophoresized at the end of treatment. Electrophoresis was conducted with 7% SDS-PAGE method. Gel stained in 0.25% Coomasie Brilliant Blue and it followed densitometry on band in gel to comparing those quantitatively. The result show the effect on the level of serum protein whose MW 29. Highest stimulation of protein synthesis (2.2 times than control) have been reached at treatment with extract that equal with 3,5 mg dry root/ 100 g BW/ day. Increasing Java gingseng root extract wasn’t followed by increasing of stimulation protein synthesis level.

Published
2014-10-29
How to Cite
Nurhariyati, T., Winarni, D., Salamun, S., Manuhara, Y. S. W., & Husen, S. A. (2014). PENGARUH EKSTRAK AKAR GINGSENG JAWA DALAM BERBAGAI KADAR TERHADAP SINTESIS PROTEIN SERUM TIKUS. BERKALA PENELITIAN HAYATI JOURNAL OF BIOLOGICAL RESEARCHES, 6(1), 45-54. https://doi.org/10.23869/482
Section
Articles