ANALISIS 6 DNA REKOMBINAN DENGAN ENZIM EcoR1:

Ni Nyoman Tri Puspaningsih; Akhmaloka; Afaf  Baktir; Ami Soewandi J.S.; Y. Sriwulan M.

doi:10.23869/490

ANALISIS 6 DNA REKOMBINAN DENGAN ENZIM EcoR1

Ni Nyoman Tri Puspaningsih FMIPA Universitas Airlangga
Akhmaloka FMIPA Institut Teknologi Bandung
Afaf Baktir FMIPA Universitas Airlangga
Ami Soewandi J.S. FMIPA Universitas Airlangga
Y. Sriwulan M. FMIPA Universitas Airlangga

DOI: https://doi.org/10.23869/490

Keywords: amylase gene, cloning, EcoR1 digestion

Abstract

Amylase enzyme from Endomycopsis fibuligera capable to hidrolize starch into glucose. Insertion of amylase gene from Endomycopsis fibuligera into yeast (Saccharomyces cereviceae) will be able to increase the function of yeast (S.cereviceae) to digest more cheaper substrate, like starch. Before cloning in yeast, recombinant DNA will be made and analyzed in Escherichia coli strain DH5a. The result showed that the sixth transformant consist recombinant DNA that were sensitive to tetracycline medium. Analysis by EcoR1 digestion showed that the size of insertion fragment into Ycp 50 vector are around 0.3 untill 16 kb.

Accepted Ni Nyoman Tri Puspaningsih 3(1) 1997

Published

2014-01-30

How to Cite

Puspaningsih, N. N. T., Akhmaloka, Baktir, A., J.S., A. S., & M., Y. S. (2014). ANALISIS 6 DNA REKOMBINAN DENGAN ENZIM EcoR1. BERKALA PENELITIAN HAYATI JOURNAL OF BIOLOGICAL RESEARCHES, 3(1), 1. https://doi.org/10.23869/490

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Issue

Vol 3 No 1 (1997): June 1997

Section

Articles