ISOLASI KARAKTERISASI DAN UJI EKSPRESI GEN ALP1 DI Escherichia coli DH5a:

Ni Nyoman Tri Puspaningsih; Akhmaloka; Sofyan Hadi; Y. Sri Wulan Manuhara; Bambang Irawan

doi:10.23869/517

ISOLASI KARAKTERISASI DAN UJI EKSPRESI GEN ALP1 DI Escherichia coli DH5a

Ni Nyoman Tri Puspaningsih Biologi, FMIPA, Universitas Airlangga
Akhmaloka FMIPA, Instittut Teknologi Bandung
Sofyan Hadi Biologi, FMIPA, Universitas Airlangga
Y. Sri Wulan Manuhara Biologi, FMIPA, Universitas Airlangga
Bambang Irawan Biologi, FMIPA, Universitas Airlangga

DOI: https://doi.org/10.23869/517

Keywords: ALP1 gene, Direct screening PCR, gene expression

Abstract

Amylase gene (ALP1) insertion in YCp 50 cloning vector was detected by Direct Screening PCR. Result of Direct Screening PCR showed one transformants which assumed have amylase gene insert. Characterization of the recombinant DNA by Stu1 and EcoR1 restriction enzymes indicated nucleotide sequence of insert gene. Digestion by BamHI, Cla1, and EcoRV restriction enzymes showed only one band that assumed size of insert gene is about 1500 bp. Gene expression showed that amylase enzyme activity by using Somogyi-Nelson method was 88.0265 Unit. This activity was 10% higher than transformant control (Escherichia coli DH5a which content YCp50).

Accepted Ni Nyoman Tri Puspaningsih 4(2) 1999

Published

2014-01-30

How to Cite

Puspaningsih, N. N. T., Akhmaloka, Hadi, S., Manuhara, Y. S. W., & Irawan, B. (2014). ISOLASI KARAKTERISASI DAN UJI EKSPRESI GEN ALP1 DI Escherichia coli DH5a. BERKALA PENELITIAN HAYATI JOURNAL OF BIOLOGICAL RESEARCHES, 4(2), 61-67. https://doi.org/10.23869/517

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Issue

Vol 4 No 2 (1999): June 1999

Section

Articles