DETEKSI Streptococcus Sp PADA Clarias gariepinus MENGGUNAKAN AMPLIFIED ENZYME-LINKED IMMUNOSORBENT ASSAY

DETEKSI Streptococcus Sp PADA Clarias gariepinus MENGGUNAKAN AMPLIFIED ENZYME-LINKED IMMUNOSORBENT ASSAY

  • Sri P. Astuti Wahyuningsih Jurusan Biologi, FMIPA, Universitas Airlangga
  • R. Warsito Fakultas Kedokteran Hewan, Universitas Gadjah Mada
  • Hastari Wuryastuti Fakultas Kedokteran Hewan, Universitas Gadjah Mada
  • Kamiso H.N. Jurusan Perikanan, Fakultas Pertanian, Universitas Gadjah Mada
DOI: https://doi.org/10.23869/525
Keywords: Streptococcus sp., Clarias gariepinus, enzyme-linked immunosorbent assay

Abstract

An enzyme-linked immunosorbent assay has been developed which detect Streptococcus sp. Twenty Clarian gariepinus at the age of two weeks was soaked in Streptococcus sp. suspension with concentration of 108 bacteria per ml for two hours. Specimens such as blood, mucous, muscles, heart, kidney, liver, intestines and gill were collected, and assayed for the presence of Steptococcus sp. using ELISA. Result of present study showed that ELISA can be applied to detect an isolated Streptococcus sp. from tissues of Clarias gariepinus. Kidney is a primary target organ (predilection). Therefore, the kidney is a specimen of choice for diagnosis approach (es) of Streptococcus sp. infection.

Published
2014-02-18
How to Cite
Wahyuningsih, S. P. A., Warsito, R., Wuryastuti, H., & H.N., K. (2014). DETEKSI Streptococcus Sp PADA Clarias gariepinus MENGGUNAKAN AMPLIFIED ENZYME-LINKED IMMUNOSORBENT ASSAY. BERKALA PENELITIAN HAYATI JOURNAL OF BIOLOGICAL RESEARCHES, 5(1), 23. https://doi.org/10.23869/525
Issue
Vol 5 No 1 (1999): December 1999
Section
Articles