Anti-Hepatitis C virus activity of Garcinia lattissima Miq. stem barks methanol exctract

  • Dadan Ramadhan Apriyanto Department of Parasitology, Immunology, and Microbiology, Faculty of Medicine, Universitas Swadaya Gunung Jati, Cirebon 45132, Indonesia
  • Sri Hartati Research Center for Chemistry, National Research and Innovation Agency, Serpong 15310, Indonesia
  • Beti Ernawati Dewi Department of Microbiology, Faculty of Medicine, Universitas Indonesia, Jalan pengangsaan Timur 16, Jakrta10320, Indonesia
Keywords: anti-HCV, Garcinia lattissima, hepatitis c virus

Abstract

Herbal medicine treatment for Hepatitis C Virus (HCV) infection is a promising alternative to common medical HCV treatments because it has low unwanted side effects and production costs. This study aimed to evaluate the methanol extract of Garcinia lattissima stem barks as an antiviral compound against strain JFH1 (HCV) genotype 2a. Garcinia lattissima stem bark methanolic  extract (GL-SB) was added to Huh7it-1 cells infected with JFHI. Anti HCV activity was determined by Focus-forming unit (FFU) assay and the cytotoxicity activity was analyzed using the 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) assay. The GL-SB showed its efficacy as the anti-HCV agent with a 50% cytotoxicity concentration (CC50) of 34.2 µg/mL and a 50% effective concentration (EC50) of 4.7 µg/mL. The co-addition and post-infection phases of GL-SB showed an anti-HCV activity, according to a time-of-addition investigation. These findings imply that GL-SB might be a promising candidate for add-on therapy in the treatment of HCV infections.

Published
2023-12-18
How to Cite
Apriyanto, D. R., Hartati, S., & Ernawati Dewi, B. (2023). Anti-Hepatitis C virus activity of Garcinia lattissima Miq. stem barks methanol exctract. BERKALA PENELITIAN HAYATI JOURNAL OF BIOLOGICAL RESEARCHES, 29(3), 95-98. Retrieved from https://ojs.berkalahayati.org/index.php/jurnal/article/view/741