ISOLASI DAN KARAKTERISASI ENZIM b-1,3-ENDOGLUKANASE DARI TANAMAN KUBIS (Brassica oleracea cv. Capitata L.)

ISOLASI DAN KARAKTERISASI ENZIM b-1,3-ENDOGLUKANASE DARI TANAMAN KUBIS (Brassica oleracea cv. Capitata L.)

  • Y. Sri Wulan Manuhara Departemen Biologi, Fakultas Sains dan Teknologi, Universitas Airlangga
DOI: https://doi.org/10.23869/261

Abstrak

Isolation and characterization of β-1,3-endoglucanase from cabbage (Brassica oleracea var. capitata L.) have been done. It showed 40° C of optimum temperature, and optimum pH is 7. After the purification with hydrophobic interaction chromatography and ion exchange chromatography, it’s activity was increased. Based on SDS-PAGE analysis, β-1,3-endoglucanase have molecular weight around 48 kD. Antifungal activity of β-1,3-endoglukanase show that it has best inhibition zone on Fusarium solanii at extract from ion exchange chromatography.

Diterbitkan
2012-01-31
##submission.howToCite##
Manuhara, Y. S. W. (2012). ISOLASI DAN KARAKTERISASI ENZIM b-1,3-ENDOGLUKANASE DARI TANAMAN KUBIS (Brassica oleracea cv. Capitata L.). JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI, 15(2), 99-105. https://doi.org/10.23869/261
Terbitan
Vol 15 No 2 (2010): June 2010
Bagian
Articles