CHITINOLYTIC BACTERY ACTIVITY ISOLATED FROM THE MUD FIELDS

CHITINOLYTIC BACTERY ACTIVITY ISOLATED FROM THE MUD FIELDS

  • Nuniek Herdyastuti Department of Chemistry, Surabaya State University, Jl. Ketintang Surabaya 60231
  • Sari Edi Cahyaningrum Department of Chemistry, Surabaya State University, Jl. Ketintang Surabaya 60231
  • Tri Joko Raharjo Department of Chemistry, Gadjah Mada University, Sekip Utara Yogyakarta, 55281
  • Mudasir Department of Chemistry, Gadjah Mada University, Sekip Utara Yogyakarta, 55281
  • Sabirin Matsjeh Department of Chemistry, Gadjah Mada University, Sekip Utara Yogyakarta, 55281
DOI: https://doi.org/10.23869/262

Abstrak

A chitinolytic microorganism had been isolated from mud fields in Ketintang Surabaya area by using a minimal media containing 0.4% colloidal chitin. Activity assay is based on released N-acetyl-glucosamine which reacted with 3,5 dinitrosalicyclic acid. Mud fields produce 63 bacterial isolates with chitinase activity (TNH1 – TNH63 isolates). The highest activity was shown by TNH11 isolate with specific activity 1.27 U/mg. TNH11 isolates was Gram negative, rod-cocoid cell, has a colony of yellow, round shape, convex elevation, flat margin and the colony diameter 3–5 mm.

Diterbitkan
2012-01-31
##submission.howToCite##
Herdyastuti, N., Cahyaningrum, S. E., Raharjo, T. J., Mudasir, & Matsjeh, S. (2012). CHITINOLYTIC BACTERY ACTIVITY ISOLATED FROM THE MUD FIELDS. JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI, 15(2), 107-111. https://doi.org/10.23869/262
Terbitan
Vol 15 No 2 (2010): June 2010
Bagian
Articles